Autumn meeting, 10th october 2017
Location: Charles River, ‘s Hertogenbosch
World leader in animal-free innovations by 2025: that is the aim of the Dutch State Secretary of Economic Affairs van Dam. On April 8, 2016, he asked the Netherlands National Committee for the protection of animals used for scientific purposes (NCad) to draw up a schedule for the phasing out of animal procedures. According to NCad, the use of laboratory animals in regulatory safety testing of chemicals, food ingredients, pesticides and (veterinary) medicines could be phased out by 225, whilst maintaining the existing safety level.
In this meeting we focus on the state of affairs with regard to the implementation of alternative methods for one of the regulatory endpoints in toxicological safety testing of chemicals: sensitization. We will discuss the testing strategy, proposed by ECVAM, which is based on three alternative OECD-tests that cover three important key events in the OECD Adverse Outcome Pathway for sensitization: the direct peptide reactivity assay (DPRA)(OECD TG 442C), the KeratinoSensTM or LuSens assay (OECD TG 442D) and the human cell line activation test (h-
CLAT)(OECD TG 442E). How does this testing strategy look like and what is its regulatory status?
Which are the important features of the proposed tests and what experience is available so far?
What can be concluded regarding the validity of the results of the testing strategy?
Here you will find the presentations held during this meeting.
Janine Ezendam (RIVM): Regulatory need for non-animal approaches: presentation on the development of
OECD test guidelines and guidance on how to design a testing strategy to combine these methods
for hazard identification and potency assessment.
Walter Westerink (CRL): Non-animal skin sensitization testing under REACH
Non-animal skin sensitization testing under REACH Walter Westerink
David Hart (AkzoNobel): In-vitro Skin sensitisation testing – What are the benefits/limitations
Experience with In-vitro skin sensitisation tests 10th October 2017